Bring the pouch to room temperature (15-30°C) before opening it. Remove the cassette from the sealed pouch and use it as soon as possible.
Place the cassette on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approximately 120 ul) to the specimen well of the cassette, and then start the timer. Avoid trapping air bubbles in the specimen well.
Wait for the colored line(s) to appear. The result should be read at 3 minutes.
NOTE: A low hCG concentration might result in a weak line appearing in the test line region (T) after an extended period of time; therefore, do not interpret the result after 10 minutes.